Sterilisation & Care

Water Quality and Spotting in Autoclaves

Why the water you use in your autoclave and final rinse matters for spotless instruments.

Water Quality and Spotting in Autoclaves
In this article
  1. Why water quality matters
  2. Recommended water
  3. Where water matters
  4. Other causes of spotting
  5. Maintenance
  6. Removing existing spots

White spots, chalky films and stains on freshly sterilised instruments are often caused by water quality rather than the instruments or the autoclave. Fixing the water source usually fixes the problem.

In this guide: Why water quality matters, Recommended water, Where water matters, Other causes of spotting, Maintenance, Removing existing spots
What this guide covers.

Why water quality matters

Tap water contains dissolved minerals such as calcium and magnesium, and sometimes chlorides. When water evaporates from instruments during drying, these minerals are left behind as spots. Chlorides can also cause pitting corrosion over time.

Most autoclave manufacturers specify distilled, deionised or reverse-osmosis (RO) water. Check your autoclave manual for the required water quality.

Where water matters

  • Autoclave reservoir — always use the specified water.
  • Final rinse after cleaning — rinsing with treated water prevents mineral spots.
  • Ultrasonic solution — using treated water helps the detergent work correctly.

Other causes of spotting

  • Detergent residues not rinsed off.
  • Instruments not fully dry before sterilisation.
  • Autoclave not cleaned or drained as recommended.
  • Overloaded chamber preventing proper drying.

Maintenance

  1. Drain and clean the reservoir as the manufacturer recommends.
  2. Clean the chamber and door seal regularly.
  3. Replace filters on schedule.
  4. Keep a maintenance log.
Key points: Where water matters
Key points: Where water matters.

Removing existing spots

Mineral spots can usually be removed with an instrument cleaner designed for stainless steel. Avoid abrasive pads that scratch the surface.

See also: preventing rust and staining.

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